Benzalacetone synthase
نویسندگان
چکیده
منابع مشابه
Benzalacetone Synthase
Benzalacetone synthase, from the medicinal plant Rheum palmatum (RpBAS), is a plant-specific chalcone synthase (CHS) superfamily of type III polyketide synthase (PKS). RpBAS catalyzes the one-step, decarboxylative condensation of 4-coumaroyl-CoA with malonyl-CoA to produce the C(6)-C(4) benzalacetone scaffold. The X-ray crystal structures of RpBAS confirmed that the diketide-forming activity is...
متن کاملA structure-based mechanism for benzalacetone synthase from Rheum palmatum.
Benzalacetone synthase (BAS), a plant-specific type III polyketide synthase (PKS), catalyzes a one-step decarboxylative condensation of malonyl-CoA and 4-coumaroyl-CoA to produce the diketide benzalacetone. We solved the crystal structures of both the wild-type and chalcone-producing I207L/L208F mutant of Rheum palmatum BAS at 1.8 A resolution. In addition, we solved the crystal structure of th...
متن کاملProtein preparation, crystallization and preliminary X-ray analysis of Polygonum cuspidatum bifunctional chalcone synthase/benzalacetone synthase.
The chalcone synthase (CHS) superfamily of type III polyketide synthases (PKSs) generate the backbones of a variety of plant secondary metabolites. An active bifunctional chalcone synthase/benzalacetone synthase (CHS/BAS) from Polygonum cuspidatum was overexpressed in Escherichia coli as a C-terminally polyhistidine-tagged fusion protein, purified to homogeneity and crystallized using polyethyl...
متن کاملElectroreduction of Benzalacetone in N,N -dimethylformamide
The electrochemical reduction of benzalacetone (BA) has been studied at mercury cathodes in N,N-dimethylformamide solutions by the techniques of cyclic voltammetry and controlled potential coulometry. The radical anion (BA'-) forms ion pairs with tetraethylammonium cations and then disappears in oligomerization processes. Atentative structureof the dimer BA BA"-... (C2H5)4N+is suggested on the ...
متن کاملSite-directed mutagenesis of benzalacetone synthase. The role of the Phe215 in plant type III polyketide synthases.
Benzalacetone synthase (BAS) and chalcone synthase (CHS) are plant-specific type III polyketide synthases (PKSs) that share approximately 70% amino acid sequence identity. BAS catalyzes a one-step decarboxylative condensation of 4-coumaroyl-CoA with malonyl-CoA to produce a diketide benzalacetone, whereas CHS performs sequential condensations with three malonyl-CoA to generate a tetraketide cha...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 2001
ISSN: 0014-2956
DOI: 10.1046/j.1432-1327.2001.02255.x